Quantitative PCR probes - TaqMan^TM Probes

Now also available with Black Hole Quenchers - BHQ

TaqMan^TM probes are oligonucleotide probes that contains a fluorophore (5'-Fam or others) and a quencher (3'-Tamra or 3'-BHQ). As long as the fluorophore and the quencher are linked to the oligonucleotide, the fluorescence is quenched. During the amplification reaction, the 5' => 3' exonuclease activity of the Taq polymerase cleaves the fluorophore from the probe. Since the fluorophore is no longer in close proximity of the quencher, the fluorescence is emitted and can be measured. The fluorescence is in direct proportion to the amount of target DNA accumulation during the PCR cycles. TAG Copenhagen recommends the probes to be 20-29 bases long.
TaqMan^TM oligos from TAG Copenhagen are always RP-HPLC purified and quality controlled by MALDI-TOF MassSpec.

For prices please inquire.

Degenerated oligos - oligos containing mixed bases

TAG Copenhagen do not charge extra¹ for mixed bases (wobbles) when they are in equal representation (25% of each base, 33% of 3 bases or 50/50% of 2 bases). When ordering oligos containing wobbles please use the following codes IUPAC-IUB symbols:
R (A or G), Y (C or T), M (A or C), K (G or T), S (G or C), W (A or T),
H (A or C or T), B (G or T or C), V (G or C or A), D (G or T or A), N (G or A or T or C).

(1) Oligos with wobbles in 3' will be charged extra. Please inquire.

TAG Copenhagen also offers doped oligos with mixed bases in un-equal representation. For best result please limit the number of different mixed positions to 40 and limit the total sequence to 100 bases.
For price and delivery time - please inquire.