Product Delivery

TAG Copenhagen has several different synthesis scales in order to provide our customer with an amount that matches the customers demand

Scale of Synthesis	10 nmol * MiniPrimers	40 nmol Standard	200 nmol	1000 nmol	Larger
Amount without HPLC (20mer)	2 OD	4 OD	12.5 OD	70 OD	>100 OD
Expected amount with HPLC (20mer)	N/A	1 OD	3 OD	10-30 OD	>50 OD
Max. Length	30 bases	80 bases	120 bases	80 bases	80 bases
Application	Sequencing PCR	Sequencing Primer -Extension PCR Cloning	Sequencing PCR Primer Extension, Cloning, Hybridisation	Sequencing Primer Extension PCR, Cloning, Hybridisation, Diagnostics	Hybridisation, Antisense, Diagnostics

Modified oligos has 50% less yield than HPLC purified oligos in same synthesis scale.

* MiniPrimers (10nmol scale) are not allowed with any modifications.

Long oligos with HPLC purification will have a lower expected yield than shorter HPLC purified oligos.

Fixed Standard Concentration - another option free of charge from TAG Copenhagen

You can choose to have the primers delivered in a Fixed concentration of 20 µM, 50 µM or 100 µM.

Primers ordered in a Fixed 20 µM or 50 µM are both synthesised in 0.01 µmol scale at our normal list price of DKK 3 each base.

Primers in a Fixed 100 µM concentration are synthesised in 0.04 µmol scale at our normal list price of DKK 4 each base.

This offer is valid only for primers up to 30 bases with no modifications. All primers in Fixed Standard Concentration will be purified by Reverse Phase Column Purification and will be delivered in dry form. Add 100 µl of water and your primers are ready to use in the requested concentration.

Documentation
All oligos from TAG Copenhagen are delivered quantified and fully documented. All data will appear on an Analytical Report and on the Vial label. TAG Copenhagen was one of the very first companies in the world to include concentration on the Vial Label.

Calculations used in the Documentation
The formulas used for the calculation in our report are as follows:

Calculation of Extinction Coefficient (e)
e = nA*15.30 + nC*7.40 + nG*11.80 + nT*9.30

Molecular Weight of DNA
MW = nA*313.2+nC*289.2+nG*329.2+nT*304.2-62
MWas = MW + (nA+nC+nG+nT-1) 16.06

Concentration 
µg/OD = MWas/e
n moles/OD = 1000/e

Melting Temperature
Tm = 69.3 + GC%*0.41 - 650/(nA+nC+nG+nT)

Simplified general calculations
Molecular weight of an oligonucleotide = Length x 330
i.e. for a 20mer, molecular weight = 6600
1 OD unit per 33 µg is an average figure for absorbance, therefore 5 OD = 165 µg